Antibacterial activities of rosemary (Rosmarinus officinalis L.) essential oil and ethanol extract

Yeddes Walid 1, 2, Hammami Majdi 1, Khammassi Saber 1, Grati Affes Taycir 1, 3, Aidi Wannes Wissem 1, * and Saidani-Tounsi Moufida 1

1 Laboratory of Aromatic and Medicinal Plants, Borj Cedria Biotechnology Center, BP901, 2050 Hammam-Lif Tunisia.
2 Faculty of Science of Bizerte, University of Carthage, Jarzouna 7021 Tunisia.
3 LR/BPIA Institute National Agronomique de Tunisie (INAT), Tunis-Mahrajène, Université de Carthage, Tunisie.
 
Research Article
Open Access Research Journal of Multidisciplinary Studies, 2022, 03(01), 001–008.
Article DOI: 10.53022/oarjms.2022.3.1.0073
Publication history: 
Received on 01 December 2021; revised on 05 January 2022; accepted on 07 January 2022
 
Abstract: 
The purpose of this study was to determine the chemical composition and antibacterial potential of rosemary essential oil and ethanol extract to improve its physicochemical and antibacterial properties. the essential oil yield of rosemary leaves was 1.76 % and the predominant compounds were 1,8-cineole (57.55 %), camphor (8.82 %), α-pinene (8.83 %), borneol (5.54 %) and camphene (2.36 %). Rosemary extract had an important amount of total phenolic content with 28.47 mg GAE/g DW. However, flavonoids and condensed tannins were weakly present in rosemary extract having 2.59 mg QE/g and 1.02 mg CE/g, respectively. Rosemary ethanol extract was mainly characterized by the predominance of carnosic acid (58.71 mg /g) and rosmarinic acid (16.51 mg/g). The antibacterial activity of rosemary essential oil and ethanol extract was determined against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Campylobacter jejuni, Salmonella enterica, Bacillus subtilis and Enterococcus faecalis.
 
Keywords: 
Rosemary leaves; Ethanol extract; Essential oil; Antibacterial activity
 
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