Dual morphometric and microcellular spatial distribution analysis during zone-specific callogenesis in Fouquieria splendens
1 Plant Physiology Laboratory, Botany Department, School of National Biological Sciences, National Polytechnic Institute, México City 11340, México.
2 Variation and Evolution Laboratory, Zoology Department, School of National Biological Sciences, National Polytechnic Institute, México City 11340, México.
Research Article
Open Access Research Journal of Life Sciences, 2026, 12(01), 018–027.
Article DOI: 10.53022/oarjls.2026.12.1.0027
Publication history:
Received on 30 June 2026; revised on 04 August 2026; accepted on 06 August 2026
Abstract:
Plant growth, development, and physiology had a complex process across scales from molecular, cellular, tissue, and whole organism. Particularly, the plant in vitro studies have the attention on individual aspects of plant development. Mathematical and computational analysis are powerful tools that allow the integration of experimental data associated to in vitro mophogenesis of plants by image processing techniques. This work characterized the morphometric features of selected development zones of Fouquieria splendens callus by the analysis of scanning electron microscopy images and the spatial distribution of cells to describe the length and position of them on callogenesis process. Principal Component Analysis (PCA) successfully discriminated the developmental zones, accounting for 73.34% of the total explained variance and revealing that callogenesis is governed by discrete, predictable morphogenetic transitions with a coordinated and hyper-regular microcellular dispersion across all evaluated regions that effectively mitigate chaotic or disordered growth. Also, macrostructural descriptors showed homeostatic stabilizing agents that restore tissue density homogeneity. Finally, the fixed geometric canalization observed in roundness and aspect ratio parameters confirms that mechanical and structural stimuli strictly regulate cellular shape prior to massive volumetric cell expansion processes showing together the in vitro cellular mass development in this xerophytic species.
Keywords:
Fouquieriaceae, Callus Culture; Scanning Electron Microscopy; Morphometrics
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