A comparative studies on the effect of two types of laser on growth, chemical composition and biochemical genetic markerson Tagetesofficinalisplant

Sami Ali Metwally 1, *, BedourHelmy Abou-Leila 2, NermeenMahdy Taha 1 and Rashad Mahmoud Shoaib 3

1 Department of Ornamental Plant and Woody Trees.
2 Department of Water Relation and Field Irrigation.
3 Department Genetics and Cytology National Research Centre, Dokki, Cairo, Egypt.
 
Research Article
Open Access Research Journal of Life Sciences, 2022, 03(02), 042–052.
Article DOI: 10.53022/oarjls.2022.3.2.0039
Publication history: 
Received on 03 April 2022; revised on 13 May 2022; accepted on 16 May 2022
 
Abstract: 
Background: Tagetes plant Calendula officinalis, (Marigold) was grown widely used as annual, perennial and herbaceous ornamentalplant belongs to the family of asteraceae. It is anusually economic plant species utilized in processed forms in modern medicinal industry. Phenolic compounds, carbohydrates, lipids, steroids, tocopherols, terpenoids, vitamin C, carotenoidsare the main constituents of Marigold.Laser rays have attained much attention at different parts of the world for improving growth and quality of plants. In this concern, Laser treatments can modify important components of plant cell and have been reported to affect differentially, the morphology, anatomy, biochemistry and physiology of plant depending on the source and time of laser exposure.
Results:The results indicated that plant height (cm), branches number/plant, stem diameter (mm), root length (cm), flowers number, plant fresh and dry weight, chlorophyll a, chlorophyll b, carotenoids, anthocyanine flowers contents, and electrical conductivity recorded significant increase at 20, 40 min. exposure time of helium-neon and cadmium laser rays. On the contrary, the previously mentioned treatments recorded decrement onanthocyanine stem contents, days from planting to flowering (earlying the flowering), and carbohydrates content. Moreover, the data of SDS PAGE demonstrated, and the effect of laser beam increased proportionally with laser exposure time.
Conclusions: From results it could be concluded that, the high exposure time (40 min.) of laser beam can be used to enhancing growth parameters, chemical constituents and exchange gene expiration which enhances a new protein formulation may provide a better alternative of petals.
 
Keywords: 
Tagets Sp; Growth; Laser Rays; Chemical Constituents; SDS-PAGE; Gene Expiration
 
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