Phenolics and phytochemicals in methanolic extract of Peperomia pellucidia quantified by HPLC
Department of Biochemistry, Niger Delta University, Amassoma, Bayelsa State.
Research Article
Open Access Research Journal of Life Sciences, 2022, 03(02), 059–062.
Article DOI: 10.53022/oarjls.2022.3.2.0045
Publication history:
Received on 28 April 2022; revised on 03 June 2022; accepted on 05 June 2022
Abstract:
Phenolics are compounds that contributed greatly to the healing potentials of many herbs, spices and plants of which Peperomia pellucidia is inclusive. This research aimed at determining the various phenolics present in the methanolic extract of Peperomia pellucidia extract. High performance liquid chromatography was used to analyse the phenolic content in Peperomia pellucidia extract. The result showed the presence of phenols and flavonoid quantified as total phenol 12.78 ± 2.70 mg GAE/g dry extract and total flavonoid as 10.97 ± 0.00 mg QE/g dry extract. HPLC result reveals the following phenols p-coumaric acid 32.76 mg/100g, gallic acid with a concentration of 138.07 mg/100 g. caffeic acid and naringenin were also detected in larger concentration as showed in the table. Ferulic acid, syringic acid and quercetin each has a retention time of 17.09, 17.47 and 22.60 min, a percentage area of 136.77, 96.93 and 91.06 while the concentrations where 16.129 mg/100g, 17.688 and 75.56 mg/100g respectively. Other phenolics with much higher quantity are rosmarinic acid and chlorogenic acid with retention time 23.97 and 25.06, a percentage area of 94.44 and 24.48, while the amounts quantified in Peperomia pellucidia extract was 19.133 mg/100g and 28.865 mg/100g. In conclusion methanolic extract of Peperomia pellucidia showed a lot of phenols that are very useful in the alleviation of stress that is linked with many diseases.
Keywords:
Peperomia pellucidia; Phenols; HPLC; Phytochemicals; Flavonoid
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