Histological investigation for the effect of non-steroidal anti-inflammatory drug [diclofenac] on myocardium and lung of local breed Rabbit
1 Department of Biology, College of Education for women, University of Kirkuk, Iraq.
2 Department of pharmacology, Physiology and Biochemistry, College of Veterinary Medicine, University of Tikrit, Iraq.
Research Article
Open Access Research Journal of Life Sciences, 2024, 08(02), 046-054.
Article DOI: 10.53022/oarjls.2024.8.2.0038
Publication history:
Received on 03 November 2024; revised on 17 December 2024; accepted on 19 December 2024
Abstract:
Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used as analgesics, anti-inflammatory agents, and antipyretics. The goal of this study was to perform a histological assessment of therapeutic and overdose dosages of diclofenac on the heart and lung tissues and serum Troponin I, Lactate Dehydrogenase, and Creatine Kinase. Thirty local breed rabbits weighing 1000 and 1400 grams were used, divided into four groups: therapeutic group I, overdose group II, overdose group III, and control group. Each group was injected intraperitoneum with different doses of diclofenac (2, 20, 50 mg/kg) once daily for 20 days, while the control group was injected with normal saline. The results of heart sections in the experimental groups showed numerous histological alterations. In group I, muscle fibers were degenerated, lipid droplets within hemolyzed, and fibrinoid deposition in congested blood vessels. Group II showed a woven appearance of muscle fibers, atrophied muscle fibers, and an increased presence of fibroblasts between blood vessels and adjacent muscle fibers. In group III, colloid appearances, pavement of inflammatory cells on the tunica intima of a congested blood vessel, muscle fiber necrosis, and hemosiderin deposits were observed between muscle fibers. The lung results referred to that group I had thickened the interstitial connective tissue, hyperplasia in connective cells, and cytoplasmic vacuolation of type I alveolus cells. In group II, the results revealed folded alveolar walls, droplets in hemolyzed congested blood vessel, and aggregation of desquamated cells within the lumen of alveoli. Group III showed engorged pulmonary vessel, folded alveolar walls surrounded with inflammatory cells infiltration. The results of serum Troponin I, creatine kinase and Lactate dehydrogenase showed a significant increase in between the experimental group when compared with control group, otherwise, the results of Troponin I in group III showed a significant decrease when compared with the control group on level 0.05%. Finally, the results of serum cholesterol a significant increase in between experimental when compared with control group on level 0.05%.
Keywords:
Nonsteroidal anti-inflammatory; Diclofenac; Troponin I; Creatine kinase and heart muscle fibers
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