Comparative study of bioremediation of crude oil spillage using Cissus populnea stem and root as bio-sorbents
1 National Center for Technology Management (NACETEM), Modibbo Adama University (MAU) Yola, Adamawa State, Nigeria.
2 Department of Chemistry, Modibbo Adama University Yola, P.M.B. 2076, Adamawa State, Nigeria.
Research Article
Open Access Research Journal of Life Sciences, 2023, 05(02), 021–026.
Article DOI:10.53022/oarjls.2023.5.2.0025
Publication history:
Received on 08 March 2023; revised on 01 May 2023; accepted on 04 May 2023
Abstract:
The total petroleum hydrocarbon concentration decrease at the initial and final bioremediation across different variable and the comparative effect of roots and stem of Cisuss populnea biosorbent were studied. 25 g of soil was weighed into different plate, contaminated with about 10 ml of crude oil, the plate was labeled as BI, CI DI, BF, CF and DF (stem), (root) across 3 g, 6 g, and 9 g variables and A (soil and crude oil mixture) as control for initial and final bioremediation. 1 g of each contaminated soil sample was extracted using mechanical shaker at room temperature and the extracts were analyzed using GC/MS the results showed that the initial treatment of stem biosorbent were 358.47455 ppm, 248.02045 ppm, 249.97273 ppm respectively and the final treatment after 4 weeks of biodegradation were 173.40636 ppm, 70.44364 ppm, 45.60818 ppm and control 757.39864 ppm. The total effect removal of Total petroleum hydrocarbon obtained were 51.6%, 75.2% and 81.8%, while the initial treatment of the root biosorbent were 366.72762 ppm, 347.75909 ppm, 337.04227ppm, after 4 weeks of biodegradation, the Total petroleum hydrocarbon concentration decreases to 143.6982 ppm, 60.05455 ppm, 52.21045 ppm and control 757.39864 ppm and the effect removal were 60.8%, 82.7% and 84.5%.
Keywords:
Bioremediation; Crude oil; Cissus populnea; Stem; Root
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