The chemistry of Crismer’s test for glucose in urine
Organic Chemistry Department, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City (CDMX), Mexico.
Research Article
Open Access Research Journal of Chemistry and Pharmacy, 2021, 01(02), 005–008.
Article DOI: 10.53022/oarjcp.2021.1.2.0028
Publication history:
Received on 14 October 2021; revised on 13 November 2021; accepted on 15 November 2021
Abstract:
The study of what is going on in a chemical test is important, the more if the involved substance is glucose in urine. In the Crismer’s test the blood-red colour of safranin solution in alkaline medium is discharged if the glucose concentration is higher than 100 mg/dl. The reagent is not affected by uric acid or creatinine as is the case with the Fehling solution. The safranin test detects sugar in saliva when other reagents failed. Since the reaction mechanism of this test has not been advanced, we provide the electron flow, step by step.
Keywords:
Glucose; Oxido-reduction; Phenazinium; Reaction mechanism; Safranin; Urine test
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